ENZYMOLOGY 2 (BCH 410) | NAIJALEARNHUB

About Course

This course teaches the concept behind enzymes and their interaction with substrates and other regulatory molecules. In this course you would understand the mechanism of action of enzymes, to include the kinetics of bisubstrate reactions, transient kinetics and the effect of pH and temperature on enzyme catalyzed reactions. You would also learn detailed treatment of allosteric behavior of enzymes, the concept of equilibrium binding and the scatchard plot.

Concept behind enzymes
Mechanism of action of enzymes
Enzyme interaction with substrates
Kinetics of bisubstrate reactions
Transient kinetics
Effect of temperature on enzyme catalyzed reaction
Detailed treatment of allosteric behavior of enzymes
Concept of equilibrium binding
Scatchard plot.

Course Content

MODULE 1 – INTRODUCTION TO ENZYMOLOGY
LEARNING GOALS: To understand the unique properties of enzyme catalysed reactions and also with respect to their active sites and regulatory sites; To be able to identify the active site amino acid groups in an enzyme.

Introduction to Enzymology

38:44
Identifying the active site groups

20:51
Summary

11:06

MODULE 2 – MECHANISM OF ACTION OF SOME KNOWNED ENZYMES
LEARNING GOALS: Describe the role of the amino acid residue in the active site of chymotrypsin, ribonuclease A, lysozyme, glutathione reductase and alcohol dehydrogenase. You should also be able to identify and explain the catalytic mechanism of the aforementioned enzymes including pyruvate dehydrogenase complex.

Types of catalytic mechanism

16:15
Bovine Pancreatic Ribonuclease A

19:56
Scheme showing the catalytic mechanism of Bovine Pancreatic Ribonuclease A
Serine Proteases

01:12:10
Scheme showing the catalytic mechanism of Chymotrypsin (Serine Protease)
Hen Egg White Lysozyme

01:02:07
Scheme showing the catalytic mechanism of lysozyme
Glutathione Reductase

41:47
Alcohol Dehydrogenase

09:06
Pyruvate Dehydrogenase Complex

27:16
Summary of Pyruvate Dehydrogenase Complex

13:53

MODULE 3 – MECHANISM AND KINETICS OF BISUBSTRATE REACTIONS
LEARNING GOALS: To be able to differentiate the types of Bisubstrate reactions; To be able to identify & estimate the kinetic parameters (KsA, KmA, KmB and Vmax) from primary and secondary plots for reaction mechanism obeying the general rate equation; To prove that the Alberty rate equation obeys the Michaelis Menten equation; To show the relationship between the Dalzier equation and the Alberty rate equation; To understand how the use of primary plots, product inhibition studies and isotope exchange at equilibrium can be used to diagnose the reaction mechanism employed by a bisubstrate enzyme.

MODULE 4 – TRANSIENT KINETICS, EFFECT OF pH AND TEMPERATURE ON ENZYME ACTIVITY
LEARNING GOALS: To be able to describe the technique used to measure the rates of an enzyme catalysed reaction and to be able to describe and estimate the effect of pH and temperature on enzyme activity.

MODULE 5 – EQUILIBRIUM BINDING EQUATION AND THE SCATCHARD PLOT
LEARNING GOALS: To derive the binding equations for a dimeric and trimeric proteins of identical binding sites, and be able to analyze the equilibrium binding data using the scatchard plot.

MODULE 6 ALLOSTERIC ENZYMES
LEARNING GOALS: To explain the concept of allosterism and the behaviour of allosteric enzymes using named examples, and also to describe the models of allosterism.

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About Course

What Will You Learn?

Course Content

MODULE 1 – INTRODUCTION TO ENZYMOLOGY LEARNING GOALS: To understand the unique properties of enzyme catalysed reactions and also with respect to their active sites and regulatory sites; To be able to identify the active site amino acid groups in an enzyme.

Identifying the active site groups

Summary

Types of catalytic mechanism

Bovine Pancreatic Ribonuclease A

Scheme showing the catalytic mechanism of Bovine Pancreatic Ribonuclease A

Serine Proteases

Scheme showing the catalytic mechanism of Chymotrypsin (Serine Protease)

Hen Egg White Lysozyme

Scheme showing the catalytic mechanism of lysozyme

Glutathione Reductase

Alcohol Dehydrogenase

Pyruvate Dehydrogenase Complex

Summary of Pyruvate Dehydrogenase Complex

MODULE 4 – TRANSIENT KINETICS, EFFECT OF pH AND TEMPERATURE ON ENZYME ACTIVITY LEARNING GOALS: To be able to describe the technique used to measure the rates of an enzyme catalysed reaction and to be able to describe and estimate the effect of pH and temperature on enzyme activity.

Transient enzyme kinetics

pH and enzyme catalysed reactions

Effect of pH on enzyme activity

Effect of temperature on enzyme activity

MODULE 5 – EQUILIBRIUM BINDING EQUATION AND THE SCATCHARD PLOT LEARNING GOALS: To derive the binding equations for a dimeric and trimeric proteins of identical binding sites, and be able to analyze the equilibrium binding data using the scatchard plot.

Steady state kinetics

Equilibrium binding equation for a dimeric protein

Equilibrium binding equation for a trimeric protein

The scatchard plot and equilibrium dialysis studies

MODULE 6 ALLOSTERIC ENZYMES LEARNING GOALS: To explain the concept of allosterism and the behaviour of allosteric enzymes using named examples, and also to describe the models of allosterism.

Introduction and properties of allosteric enzymes

Allosteric modulators and cooperativity

Phosphofructokinase 1 (PFK-1)

Protein Kinase A (PKA)

Aspartate Transcarbamoylase (ATCase)

Models of allosterism

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MODULE 1 – INTRODUCTION TO ENZYMOLOGY
LEARNING GOALS: To understand the unique properties of enzyme catalysed reactions and also with respect to their active sites and regulatory sites; To be able to identify the active site amino acid groups in an enzyme.

MODULE 4 – TRANSIENT KINETICS, EFFECT OF pH AND TEMPERATURE ON ENZYME ACTIVITY
LEARNING GOALS: To be able to describe the technique used to measure the rates of an enzyme catalysed reaction and to be able to describe and estimate the effect of pH and temperature on enzyme activity.

MODULE 5 – EQUILIBRIUM BINDING EQUATION AND THE SCATCHARD PLOT
LEARNING GOALS: To derive the binding equations for a dimeric and trimeric proteins of identical binding sites, and be able to analyze the equilibrium binding data using the scatchard plot.

MODULE 6 ALLOSTERIC ENZYMES
LEARNING GOALS: To explain the concept of allosterism and the behaviour of allosteric enzymes using named examples, and also to describe the models of allosterism.